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RNA-Seq

ArrayGen > Services > Workshops / Training > RNA-Seq

     
  Good News For Researchers !!!
A workshop for PhD students and post-doctoral researchers coming with their own real data sets will get the training and their data analysis done at considerable discount. After this course they will be able to analyze their own data and create ready-to-publish graphics.

Goals :

RNA-Seq workshop is to introduce researchers to the basic principles of analyzing RNA seq data generated by next-gen sequencing approaches.The most important NGS file formats (fastq, sam/bam, bigWig, etc.) are introduced and one proceeds with first hands-on analyses (QC, mapping, visualization).

In this hands-on session we will align example data from an RNA-Seq experiment against the genome, assign the reads to genes, count the expression of genes and then check for statistical significance of differential expression.


Training Information
Period of Training Offline - 15 days Training + 15 days Project work   [ 2 Hours Daily [ Monday To Saturday ] ]
Online - 15 days Training + 15 days Project work   [ 2 Hours Daily [ Monday To Saturday ] ]
Registration Open
(Training can be started on any date any timing as per the candidate convenience)
Module - I Advanced Bioinformatics & basic programming
Topics - Introduction to Bioinformatics
- Understanding Genomics Bioinformatics
- Databases & tools (NCBI,UCSC,BLAST,BLAT etc)
- Gene Prediction
- Genome Annotation
- Genome Vizualization
- Basic Perl programming
- Unix based commands
Module - II Next Generation Sequencing (NGS) - RNASeq Data Analysis
Topics - Overview of NGS & detailed understanding
- Data Retrieval (NCBI SRA) & Introduction to data types
- Read Quality Check (FastQC & Cutadapt)
- Alignment of reads using reference Genome (Tophat)
- Visualization of mapped reads (UCSC & IGV)
- Gene Expression Quantification (Coverage,FPKM)
- Differential expression analysis( Cufflink, cuffmerge & cuffdiff )
- Different plots using CummeRbund & R
- Pathway & Gene ontology enrichment analysis
Project Work Project will be assigned based on the training course selected.
Eligibility B.Sc/B.Tech/M.Tech. / M.Sc. /Ph.D /PostDocs/Any Life Sciences in Biological Science, Computer science, Pharmacy, Agriculture, Biotechnology, Molecular biology, Medicine, clinical research and other relevant Qualification in the life sciences areas are eligible.
Certificate All the participants will receive certificates on successful completion of training
Place ArrayGen, Pune, India
Contact info@arraygen.com



    

Registration is now open please request to sign up to reserve your place! If you are interested, you can mail us at info@arraygen.com. We hope to see you in this new year. Dont miss this opportunity.



 
 
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